All procedures were approved by the Ethics Committee on Animal Research of the Keio University School of Medicine adhered to the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research, the Institutional Guidelines on Animal Experimentation at Keio University, and the Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines for the use of animals in research. C57BL/6 J mice were obtained from CLEA Japan, Inc. Five wild type adult mice without any treatment were used to compare the measurements of AL using μCT and SD-OCT. For lens power dependency experiments, five p21 mice were used for each lens power. For the comparison of LIM with FDM, five p21 mice were worn with 0 D lenses and −30 D lenses, eight p21 mice were worn with −30 D only and ten p21 mice were worn diffusers only. For the atropine treatment experiment, four p21 mice worn 0 D and −30 D lenses were treated with atropine in both eyes and four p21 mice were treated with PBS in both eyes as control. For the OKR experiment, three mice worn −30 D lenses on their right eyes for three weeks started from p21 were used. After the measurement of the OKR, the same three mice were put into general anesthesia for the ERG measurements. All mice were fed with normal chew and water ad lib. Four or five mice with or without the frame were kept in one cage with approximately 50 lux background fluorescent lamp light for 12 h from 8:00 am to 8:00 pm.
Free full text: Click here
