Renal Tissue and Podocyte Western Blot

Renal tissue samples and podocytes were used for WB experiments following our previously described procedures [24 (link)]. Antibodies: WT-1 (Abcam Cat# ab89901, RRID:AB_2043201), FSP1 (Abcam Cat# ab197896, RRID:AB_2728774), Nephrin (R and D Systems Cat# AF3159, RRID:AB_2155023), Desmin (R and D Systems Cat# AF3844, RRID:AB_2092419), GAPDH (Proteintech Group Cat# 60004-1-Ig, RRID:AB_2107436), MAP4 (Bethyl Cat# A301-489A, RRID:AB_999616, Montgomery, Texas, USA), p38 MAPK (P38) (Cell Signaling Technology Cat# 9212, RRID:AB_330713, Danvers, MA, USA), Phospho-p38 MAPK (p-P38) (Cell Signaling Technology Cat# 9211, RRID:AB_331641). Rabbit polyclonal antibodies against p-MAP4 (S787) and p-MAP4 (S737) were made in-house and validated as reported previously [24 (link)].

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Li L., Feng Y., Zhang J., Zhang Q., Ren J., Sun C., Li S., Lei X., Luo G., Hu J, & Huang Y. (2022). Microtubule associated protein 4 phosphorylation-induced epithelial-to-mesenchymal transition of podocyte leads to proteinuria in diabetic nephropathy. Cell Communication and Signaling : CCS, 20, 115.

Publication 2022

FSP1 Nephrin Desmin GAPDH

A301 Antibodies Cat 1 Desmin Gapdh Map4 Nephrin P38 mapk Podocytes Rabbit Renal Tissue

Corresponding Organization :

Other organizations : Daping Hospital, Army Medical University, Southwest Hospital, The First Affiliated Hospital, Sun Yat-sen University, Zhongshan Hospital, Fudan University, Sun Yat-sen University, Dalian Medical University, Chongqing Medical University, Second Affiliated Hospital of Chongqing Medical University

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Variable analysis

independent variables

Renal tissue samples
Podocytes

dependent variables

Protein expression levels (measured by Western blot)

control variables

Previously described procedures [24 (link)]
GAPDH (as a loading control)

positive controls

None specified

negative controls

None specified

Annotations

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