Cellular Uptake of M-PTX Nanoparticles

Cellular uptake of M-PTX NPs in OSC-19 cells was analyzed by scanning transmission electron microscopy (STEM, Hitachi High Technologies HD-2700, Hitachi High Technologies Corporation., Tokyo, Japan) [6 (link)]. Bright-field (BF) and annular dark-field (ADF) TEM images were obtained to detect M-PTX NPs taken into OSC-19 cells. Energy dispersive X-ray spectroscopy (EDX) and X-ray mapping were used to analyze the composition of the particles. All TEM images, spectra and mapping images were taken at an accelerating voltage of 200 kV.

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Nakakaji R., Umemura M., Mitsudo K., Kim J.H., Hoshino Y., Sato I., Masuda T., Yamamoto M., Kioi M., Koizumi T., Fujita T., Yokoyama U., Iida M., Sato M., Sato H., Murofushi S., Shibata S., Aoki I., Eguchi H., Tohnai I, & Ishikawa Y. (2018). Treatment of oral cancer using magnetized paclitaxel. Oncotarget, 9(21), 15591-15605.

Publication 2018

HD-2700

Cells Cellular m Scanning transmission electron microscopy Stem X ray

Corresponding Organization :

Other organizations : Yokohama City University, Cardiovascular Institute Hospital, Yokohama National University, The University of Tokyo, Konan University, Aichi Medical University, IHI Corporation (Japan)

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Variable analysis

independent variables

Cellular uptake of M-PTX NPs

dependent variables

Detection of M-PTX NPs taken into OSC-19 cells using bright-field (BF) and annular dark-field (ADF) TEM images
Composition analysis of the particles using energy dispersive X-ray spectroscopy (EDX) and X-ray mapping

control variables

Accelerating voltage of 200 kV for all TEM images, spectra and mapping images

controls

No positive or negative controls were explicitly mentioned in the provided information.

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