Visualizing V. parahaemolyticus Infection in HeLa Cells

According to a previous report (Tandhavanant et al., 2018 (link)), assays of infection of HeLa cells were conducted. HeLa cells were plated in 24-well dishes at a density of 1.5 × 10⁵ per cell, grown for 12 h, and then infected with WT, ΔvmeL, and CΔvmeL V. parahaemolyticus at MOI of 10 for 2 h. After 2 h f co-incubation, the cells were rinsed with PBS and fixed with 4% paraformaldehyde in PBS for 15 min at room temperature. 0.5% Triton-X was used to permeabilize the cells for 10 minat room temperature. Then, the cells were probed with rhodamine-phalloidin (SBS Genetech, Shanghai, China) to stain F-actin and DAPI (Beyotime, Shanghai, China) to highlight HeLa cell DNA. Images were captured using an inverted fluorescence microscope (Axio observer Z1, ZEISS).