Immunoblotting of Cell Signaling Proteins

Immunoblotting was performed as described previously^{7 (link)}. Antibodies used in this study were as follows: NUP155 (A303-934A, Bethyl Laboratories), IPOβ (ab2811, Abcam), KNTC1 (F2051-10H4, Sigma-Aldrich), hCAP-D2 (E4161-4C12, Sigma-Aldrich), IPO7 (ab88339, Abcam), CLTC (sc-271178, Santa Cruz), Cdc2-phosphorylated vimentin Ser55 (D076-3, MBL), GST (G7781, Sigma-Aldrich), GFP (A6455, Invitrogen), and HA-tag (clone 3F10, Roche). Secondary antibodies were HRP-rabbit anti-mouse IgG (H + L) (61-6520, Invitrogen) or HRP-goat anti-rabbit IgG (H + L) (65–6120, Invitrogen).

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Yokoyama T., Yukuhiro M., Iwasaki Y., Tanaka C., Sankoda K., Fujiwara R., Shibuta A., Higashi T., Motoyama K., Arima H., Yoshida K., Sugimoto N., Morimoto H., Kosako H., Ohshima T, & Fujita M. (2019). Identification of candidate molecular targets of the novel antineoplastic antimitotic NP-10. Scientific Reports, 9, 16825.

Publication 2019

ab2811, A6455 HA-tag (clone 3F10, HRP-rabbit anti-mouse IgG (H + L) HRP-goat anti-rabbit IgG (H + L)

Anti igg Antibodies Cdc2 Clone Goat Mouse Rabbit Vimentin

Corresponding Organization :

Other organizations : Kyushu University, Kumamoto University, Daiichi University of Pharmacy, Tokushima University

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Variable analysis

independent variables

Antibodies used in this study

dependent variables

Immunoblotting results

control variables

Experimental conditions as described previously in the referenced publication

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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