Flavonoid Content Determination in Samples

A colorimetric method described by Ren et al. (2020) (link) was used to analyze total flavonoid content with rutin solutions (4, 8, 12, 16 and 20 μg/mL) serving as standards. Briefly, powdered samples were extracted with 50% (v/v) methanol in an ultrasonication bath (VCX600, Sonics and Materials Inc., Newtown, CT, USA) for 90 min at room temperature, then centrifuged at 12,000 rpm for 20 min. The supernatant was collected and used to measure absorbance at 360 nm with a UV-6000 spectrophotometer. The calibration standard was 50% (v/v) methanol.

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Zhang M., Liu N., Teixeira da Silva J.A., Liu X., Deng R., Yao Y., Duan J, & He C. (2022). Physiological and transcriptomic analysis uncovers salinity stress mechanisms in a facultative crassulacean acid metabolism plant Dendrobium officinale. Frontiers in Plant Science, 13, 1028245.

Publication 2022

VCX600

Bath Colorimetric Flavonoid Methanol Rutin

Corresponding Organization :

Other organizations : Chinese Academy of Sciences, South China Botanical Garden, Qiannan Normal College For Nationalities, Beijing Botanical Garden

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Variable analysis

independent variables

Concentration of rutin solutions (4, 8, 12, 16 and 20 μg/mL)

dependent variables

Total flavonoid content

control variables

Solvent used for extraction (50% (v/v) methanol)
Extraction method (ultrasonication bath for 90 min at room temperature)
Centrifugation parameters (12,000 rpm for 20 min)
Measurement method (absorbance at 360 nm with a UV-6000 spectrophotometer)
Calibration standard (50% (v/v) methanol)

controls

Rutin solutions (4, 8, 12, 16 and 20 μg/mL) served as standards

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