Flavonoid Content Determination in Samples
Corresponding Organization :
Other organizations : Chinese Academy of Sciences, South China Botanical Garden, Qiannan Normal College For Nationalities, Beijing Botanical Garden
Variable analysis
- Concentration of rutin solutions (4, 8, 12, 16 and 20 μg/mL)
- Total flavonoid content
- Solvent used for extraction (50% (v/v) methanol)
- Extraction method (ultrasonication bath for 90 min at room temperature)
- Centrifugation parameters (12,000 rpm for 20 min)
- Measurement method (absorbance at 360 nm with a UV-6000 spectrophotometer)
- Calibration standard (50% (v/v) methanol)
- Rutin solutions (4, 8, 12, 16 and 20 μg/mL) served as standards
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