Genetic Mouse Models in Biomedical Research

Experimental procedures involving live animals were carried out in accordance with animal welfare regulations and with approval of the Regierung Oberbayern (Az 55.2-1-54-2532-187-15 and ROB-55.2–2532.Vet_02-14-157) or in accordance with the Karolinska Institutet’s guidelines for the care and use of animals in research, and were approved by the institute’s Animal Ethics Committee respectively (Ethical permit number 19462–2017). C57Bl/6J B6 albino and C57Bl/6J mice were obtained from The Jackson Laboratory (Maine, USA). Ai14 mice and Cdh5-Cre mice were obtained from the Jackson laboratory (stock number 007914 and 017968). Bbs4^-/- mice were generated in the Lupski lab and backcrossed to C57Bl/6 J (Eichers et al., 2006 (link)). Heterozygous offspring were crossed to produce homozygous Bbs4^-/- and littermate control. Pifo^wt/- mice were crossed to produce homozygous Pifo^-/- mice and littermate controls (Kinzel et al., 2010 (link)). βICKO animals were generated by crossing Pdx1-CreER mice (Zhang et al., 2005 (link)) with Ift88^loxP/loxP mice (Volta et al., 2019 (link); Davenport et al., 2007 (link)).