Scleral Vein Injection and Conjunctival Suture Protocol

Procedures for the surgical technique have been described in detail previously [11 , 12 (link)]. Briefly, a glass microneedle (33G, Hamilton) was positioned parallel to the vessel axis, inserted into the vessel lumen and a volume of 50 µL of lauromacrogol (Polyoxyethylene lauryl ether 1%; Tianyu, Shanxi, China) was dissolved in distilled water and slowly injected into the superior scleral vein of one eye during the SI induction. During the CS induction, a CS (7-0 nylon) was placed around the equator of the eye at approximately 1.5 mm behind the limbus (Additional file 2: Fig. S1). The suture was anchored by placing it below the conjunctiva, avoiding the major episcleral drainage veins evenly spaced around the globe at five to six anchor points. The congestion of the vortex veins could be prevented by the anterior position of the suture. The eyes of the experimental animals were treated with antibiotic eye drops after the operation. The control group was accepted without any disposal. Both eyes were performed with the same treatment in experimental groups.