Male mice were sacrificed by cervical dislocation, and cauda epididymides were carefully dissected and squeezed on a clean slide to harvest spermatozoa, which were incubated in M2 medium (SIGMA, M7167) for sperm parameters testing by using the IVOS II (Hamilton Thorne Inc., Beverly, MA) computer-assisted sperm analysis system64 (link). The concentration and motility of sperm could be automatically assessed by this system.
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