DAMGO-Induced Receptor Activation Assay

We used the MOR agonist [D-Ala 2 , NMe-Phe 4 , Gly-ol 5 ]-enkephalin (DAMGO; H-2535, Bachem), GABAA receptor antagonist picrotoxin (PTX, P1675, Sigma-Aldrich), PKI (6221, Tocris), KT5720 (5720, Tocris), Forskolin (F3917, Sigma-Aldrich), Cycloheximide (0970, Tocris), ZD7288 (1000, Tocris), WIN55,212-2 (1038, Tocris), and Torin 2 (4248, Tocris).
Other reagents used for making solutions were purchased from Sigma-Aldrich or Fisher Scientific.

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Muñoz B., Fritz B.M., & Attwood B. (2024). HCN1 channels mediate mu opioid receptor long-term depression at insular cortex inputs to the dorsal striatum.

Publication 2024

Corresponding Organization :

Other organizations : University School, Indiana University, Indiana University – Purdue University Indianapolis

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Variable analysis

independent variables

[D-Ala 2 , NMe-Phe 4 , Gly-ol 5 ]-enkephalin (DAMGO)
Picrotoxin (PTX)
KT5720
Forskolin
Cycloheximide
ZD7288
WIN55,212-2
Torin 2

dependent variables

Measured outcomes

control variables

Kept constant to ensure valid results

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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