Serial spectra were phase and baseline corrected and integrated using the XWinNMR software (Bruker Biospin, Coventry, UK). The integrated peak areas from the pyruvate/lactate experiments were plotted as a function of time, and apparent reaction rates were obtained by least-squares fitting of the data to the modified Bloch equations using a two-site exchange model as previously described (Hill et al, 2013a (link), 2013b (link)). All apparent rate constants for the forward reaction of conversion of pyruvate-to-lactate (kPL) are presented as a mean concentration per second normalised to the number of cells.
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Pyruvate-to-Lactate Conversion Kinetics
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Other organizations : Institute of Cancer Research, Cancer Research UK, Royal Marsden Hospital, St Thomas' Hospital, King's College London
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Variable analysis
independent variables
- None explicitly mentioned
- Apparent reaction rates for the forward reaction of conversion of pyruvate-to-lactate (kPL)
- Number of cells
- No positive or negative controls were explicitly mentioned.
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