Protein Expression Analysis Protocols

Western blot analysis and intracellular flow cytometry was performed as in (29 (link)) using antibodies to Twist1/Twist2 (Twist2C1a), HDAC1 (10E2), HDAC3 (A-3) (Santa Cruz), NOD2 (Cayman) or GAPDH (6C5; EMD Millipore).

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Sun R., Hedl M, & Abraham C. (2019). Twist1 and Twist2 induce human macrophage memory upon Chronic Innate Receptor Treatment by HDAC-Mediated Deacetylation of Cytokine Promoters. Journal of immunology (Baltimore, Md. : 1950), 202(11), 3297-3308.

Publication 2019

Twist2C1a GAPDH (6C5;

Antibodies Cayman Flow cytometry Gapdh Intracellular Twist1 Western blot analysis

Corresponding Organization : Yale University

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Variable analysis

independent variables

Twist1/Twist2 (Twist2C1a) antibody
HDAC1 (10E2) antibody
HDAC3 (A-3) antibody (Santa Cruz)
NOD2 (Cayman) antibody
GAPDH (6C5; EMD Millipore) antibody

dependent variables

Western blot analysis
Intracellular flow cytometry

control variables

The experiments were performed as in the previous study (29 (link))

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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