Chromatin immunoprecipitation (ChIP) was performed as previously described (3 (link)) using Protein A or G magnetic beads (Dynabeads, Life Technologies) to capture antibody-chromatin complexes. Antibodies used were anti-H3K4me1 (ab8895, Abcam), anti-H3K4me3 (C15410003, Diagenode), anti-H3K4ac (07-539, millipore), anti-H3K9ac (ab4441, Abcam), anti-H3K9me3 (05-1242, millipore), H3K27ac (ab4729, Abcam), and anti-H3K27me3 (07-449, millipore).
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