Collateral vessels and the surrounding adipose tissue was harvested three days post-surgery and stained for α-smooth muscle actin FITC (1:200, clone 1A4, Sigma, St. Louis, MO), and Alexa Fluor 647 anti-mouse CD206 (1:200, clone C068C2, BioLegend, San Diego, CA) as previously described43 (link). Vessel diameters were quantified for the feeding vessel, the first branch, and the second branch, and CD206+ cells were quantified as previously described43 (link). Data presented include vessel diameter measurements of the feeding collateral vessel, the first branch of the collateral vessel, and the second branch of the collateral vessel. Three mice were repeated in three mice (n = 3).
Protocol detail
Collateral Vessel Characterization
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Corresponding Organization : University of Virginia
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Variable analysis
independent variables
- Time post-surgery (3 days)
- Vessel diameters of the feeding collateral vessel, the first branch of the collateral vessel, and the second branch of the collateral vessel
- Number of CD206+ cells
- Staining protocol (α-smooth muscle actin FITC and Alexa Fluor 647 anti-mouse CD206)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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