HPLC Analysis of Bioactive Compounds

Chromatographic analysis was carried out using an Agilent 1200 high-performance liquid chromatograph coupled to an Agilent UV-Vis diode array detector (Agilent Technologies, Santa Clara, CA, USA), based on HPLC methods previously validated for fresh fruits, herbal medicines and other food products [2 (link),69 (link)].Composition of solvents, used gradient elution conditions and UV-Vis wavelengths were listed and described in Table 5, while calibration parameters for all the used analytical standards were reported in Table 6 [70 (link)].

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Donno D., Mellano M.G., Hassani S., De Biaggi M., Riondato I., Gamba G., Giacoma C, & Beccaro G.L. (2018). Assessing Nutritional Traits and Phytochemical Composition of Artisan Jams Produced in Comoros Islands: Using Indigenous Fruits with High Health-Impact as an Example of Biodiversity Integration and Food Security in Rural Development. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(10), 2707.

Publication 2018

Agilent UV-Vis diode array detector

Chromatographic analysis Food products Fruits Herbal medicines Hplc Solvents

Corresponding Organization : University of Turin

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Variable analysis

independent variables

Composition of solvents
Gradient elution conditions
UV-Vis wavelengths

dependent variables

Chromatographic analysis
Concentration of analytes (measured using analytical standards)

control variables

Agilent 1200 high-performance liquid chromatograph
Agilent UV-Vis diode array detector
Previously validated HPLC methods for fresh fruits, herbal medicines and other food products

controls

Positive controls: Analytical standards used for calibration and quantification
Negative controls: Not explicitly mentioned

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