The following reagents were purchased: maleimide-PEG5000-carboxyl-NHS from NOF Corporation (Tokyo, Japan); 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) and cholesterol (chol) from Nippon Fine Chemicals (Osaka, Japan); Alexa Fluor® 488 C5-maleimide from Life Technologies (Carlsbad, CA, USA); CPZ hydrochloride, Nys, MβCD, Cyto D, Wort, and bacitracin from Sigma-Aldrich (St Louis, MO, USA); and rhodamine-PE lipid (Rho-PE) from Avanti Polar Lipids (Alabaster, AL, USA). The required 1,5-dioctadecyl L-glutamate (Glu2C18), PEG5000-Glu2C18, PEG5000-DSPE, GGLG, and maleimide-PEG5000-Glu2C18 were synthesized in our laboratory using previously published methods.17 (link)–19 (link)
Protocol detail
Lipid-based Nanoparticle Synthesis Protocol
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Alabaster
Alexa fluor 488 c5 maleimide
Bacitracin
Cholesterol
Dspe
Glutamate
Lipids
Maleimide
Phosphoethanolamine
Rhodamine pe
Corresponding Organization :
Other organizations : Waseda University
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Variable analysis
independent variables
- Maleimide-PEG5000-carboxyl-NHS
- 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE)
- Cholesterol (chol)
- Alexa Fluor® 488 C5-maleimide
- CPZ hydrochloride
- MβCD
- Cyto D
- Bacitracin
- Rhodamine-PE lipid (Rho-PE)
- 1,5-dioctadecyl L-glutamate (Glu2C18)
- PEG5000-Glu2C18
- PEG5000-DSPE
- Maleimide-PEG5000-Glu2C18
- Not explicitly mentioned
- Not explicitly mentioned
- Not specified
- Not specified
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