CD31 Expression Analysis in NHAECs

The expression of cluster of differentiation (CD)31 on NHAECs was analyzed by flow cytometry using appropriated primary and fluorescein isothiocyanate-labelled secondary antibodies (25 (link)). Samples were analyzed using a FACSCalibur/LSR II flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA), and cell sorting was performed with a FACSVantage cell sorter (BD Biosciences). Data were analyzed using FACSDiva™ software (version 8.0.1; BD Biosciences). Gates were set according to isotype controls.

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Amara S., Alotaibi D, & Tiriveedhi V. (2016). NFAT5/STAT3 interaction mediates synergism of high salt with IL-17 towards induction of VEGF-A expression in breast cancer cells. Oncology Letters, 12(2), 933-943.

Publication 2016

FACSVantage cell sorter FACSDiva™ software

Antibodies Cell Flow cytometry Fluorescein Isothiocyanate Isotype

Corresponding Organization :

Other organizations : Tennessee State University

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Variable analysis

independent variables

Primary and fluorescein isothiocyanate-labelled secondary antibodies

dependent variables

Expression of cluster of differentiation (CD)31 on NHAECs

control variables

Isotype controls

controls

Isotype controls

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