Immunoblotting Protocol for Cellular Signaling

This has been performed as previously described [17] (link). Antibodies directed against alpha smooth muscle actin (α-SMA), transforming growth factor beta 1 (TGF-β1, 2AR2, vascular endothelial growth factor A (VEGF-A), Stromal-derived factor-1 (SDF-1) and interleukin-6 (IL-6) were purchased from Abcam (Cambridge, MA); matrix metalloproteinase 2 (MMP-2), hypoxia inducible factor-1α (HIF-1α), Akt and phospho-Akt (193H12), Erk1/2 and phospho-Erk1/2 (THR202/TYR204) from Cell Signaling (Danvers, MA), p16^INK4a and Caveolin-1 from BD Biosciences (San Jose, CA); p21 (F-5), p53 (DO-1), PTEN (A2B1) and glyceraldehydes-3-phosphate dehydrogenase (GAPDH, FL-335) were purchased from Santa Cruz (Santa Cruz, CA).

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Al-Ansari M.M, & Aboussekhra A. (2014). Caffeine Mediates Sustained Inactivation of Breast Cancer-Associated Myofibroblasts via Up-Regulation of Tumor Suppressor Genes. PLoS ONE, 9(3), e90907.

Publication 2014

TGF-β1 VEGF-A SDF-1 p16INK4a Caveolin-1

Alpha actin Antibodies Caveolin 1 Dehydrogenase Erk1 Factor 1 Gapdh Hypoxia inducible factor 1α Interleukin 6 Matrix metalloproteinase 2 P16ink4a Phosphate Pten Smooth muscle Tgf β1 Transforming growth factor beta 1 Vascular endothelial growth factor a

Corresponding Organization :

Other organizations : King Saud University, King Faisal Specialist Hospital & Research Centre

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Variable analysis

independent variables

Antibodies directed against alpha smooth muscle actin (α-SMA), transforming growth factor beta 1 (TGF-β1, 2AR2, vascular endothelial growth factor A (VEGF-A), Stromal-derived factor-1 (SDF-1) and interleukin-6 (IL-6)
Matrix metalloproteinase 2 (MMP-2), hypoxia inducible factor-1α (HIF-1α), Akt and phospho-Akt (193H12), Erk1/2 and phospho-Erk1/2 (THR202/TYR204)
P16INK4a and Caveolin-1, p21 (F-5), p53 (DO-1), PTEN (A2B1) and glyceraldehydes-3-phosphate dehydrogenase (GAPDH, FL-335)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

This has been performed as previously described [17] (link)

Annotations

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