Immunophenotypic Analysis of HCL Cells

Multiparameter flow cytometric immunophenotype was performed as described previously [18 (link)], with sIg expression analysed on CD19⁺CD11c^HI or CD19⁺CD11c^HICD103⁺ HCL cells (Table 1). Additional markers were assessed using CD25-PE (BD Biosciences, Oxford, UK), CD27-PE (BD) and CD123-PE (BD) antibodies on CD19⁺CD11c^HICD103⁺ cells (Table 1). Data were analysed using FACSDiva (BD) and FlowJo (Tree Star Inc., Ashland, OR, USA). ANXA1 detection was performed as previously described [19 (link)]. Briefly, ANXA1 was detected either by western blot or immunocytochemistry using the antibody ‘Purified mouse anti-Annexin I’, clone 29/Annexin I (BD Transduction Laboratories, Oxford, UK) (Table 1).