Construction of undA Deletion Mutant
Corresponding Organization :
Other organizations : Écologie et Biologie des Interactions
Variable analysis
- Deletion of 762 base pairs (bp) in the undA gene
- Presence of the undA deletion mutation
- Amplification of the upstream and downstream regions of undA gene with specific primer pairs
- Overlap-PCR to obtain the deleted undA gene construct
- Cloning of the deleted undA gene construct into the pAKE604 suicide vector
- Transformation of the construct into E. coli Top10 strain
- Verification of the construct by Sanger sequencing
- Introduction of the construct into E. coli S17.1 strain
- Transfer of the plasmid to MFE01 strain by biparental mating
- Selection of the double recombination event
- Confirmation of the undA deletion mutant by PCR analysis and DNA sequencing
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!