Sequencing and Analysis of Antibiotic Resistance Genes in Pseudomonas aeruginosa

The mucA gene of all PA and for quinolone resistant QRDR genes (gyrA, gyrB, parC and parE) for 43 PA isolates included in this study were amplified by PCR. Primers used for amplification and sequencing are reported in Table 1 or previously published (Cabrera et al., 2022 (link)). PCR products were sequenced by Sanger methods (Genewiz, Germany), and were analyzed by alignment with the corresponding template sequence of PAO1 mucA at GenBank (Ciofu et al., 2010 (link)). PCR was performed in a Veriti PCR Thermal Cycler (Applied Biosystems, France) for 2 min denaturation at 94°C followed by 30 cycles of 1 min at 94°C, 1 min at 60°C and 1 min at 72°C, with a final extension of 7 min at 72°C.