Docetaxel Prodrug Cytotoxicity Assay

Example 14

The docetaxel prodrug, synthesized as illustrated in FIG. 18A or B was administered to 2F2B cells in an alamar blue assay to measure metabolic activity. FIG. 22 shows a comparison between an αvβ3 targeted particle without docetaxel, an αvβ3 targeted particle with docetaxel, and an αvβ3 targeted particle with a docetaxel prodrug. As the assay progressed, the particle comprising the prodrug demonstrated a sustained suppression of cell metabolic activity, as compared to the targeted control without docetaxel.

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US11872313B2. Prodrug compositions, prodrug nanoparticles, and methods of use thereof (2024-01-16). Washington University [US]. Inventors: Gregory M. Lanza [US], Dipanjan Pan [US].

Patent 2024

Alamar blue Assay Cell Docetaxel Prodrug

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Variable analysis

independent variables

Type of particle: (1) αvβ3 targeted particle without docetaxel, (2) αvβ3 targeted particle with docetaxel, (3) αvβ3 targeted particle with a docetaxel prodrug

dependent variables

Metabolic activity of 2F2B cells

control variables

Assay conditions (e.g., cell type, incubation time)

controls

Positive control: αvβ3 targeted particle with docetaxel
Negative control: αvβ3 targeted particle without docetaxel

Annotations

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