Mycobacterium abscessus Strain Cultivation

The Mycobacterium abscessus subsp. abscessus CIP104536^T reference strain and all derived mutant strains are listed in Supplementary Table S1. Strains were grown in Middlebrook 7H9 broth (BD Difco) supplemented with 0.05% Tween 80 (Sigma-Aldrich) and 10% oleic acid, albumin, dextrose, catalase (OADC enrichment; BD Difco) (7H9^T/OADC) at 30°C or in Sauton’s medium in the presence of antibiotics, when required. On plates, colonies were selected either on Middlebrook 7H10 agar (BD Difco) supplemented with 10% OADC enrichment (7H10^OADC) or on LB agar. For drug susceptibility testing, cultures were grown in Cation-Adjusted Mueller-Hinton Broth (CaMHB; Sigma-Aldrich). The TAC analogs D6, D15, and D17 were synthesized as reported previously (Coxon et al., 2013 (link)) and dissolved in DMSO. Other antibiotics were purchased from Sigma-Aldrich.