Iodination of Monoclonal Antibody AA98

The modified iodination protocol of AA98 mAb with ¹²⁵I was employed fundamentally as described previously (Visser et al., 2001 (link); Tijink et al., 2009 (link)). Briefly, 800 μg AA98 mAb (80 μL, 10 mg/mL) was added into a tube coated with 25 μg Iodogen (Pierce Biotechnology), then 0.5-μL Na¹²⁵I solution (GMS Pharmaceutical Co., Ltd., Shanghai, China) with specific activity of 1.6 Ci/mL was added. After 10 min, the reaction was terminated. The radiochemical purity (RCP) of ¹²⁵I-AA98 mAb was assessed by radio-thin layer chromatography (radio-TLC) Imaging Scanner (Eckert & Ziegler Radiopharma, Inc., USA).