The percent EE was determined as reported previously.37 ,39–42 (link) Briefly, 20 mg of PLGA+chitosan+IL-10 nanoparticles were mixed with NaOH and sodium dodecyl-sulfate (SDS) overnight on a rocker at room temperature (RT). Nanoparticles were centrifuged, and supernatants were obtained to measure the released IL-10 using the microBCA protein kit (ThermoFisher, Waltham, MA). EE calculation is shown below with total IL-10 (A) and non-encapsulated IL-10 (B).
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$${{\rm EE\ }}\left({{\rm \% }} \right){{\rm \ =}}\left[{{{{{\rm A - B}}} \over {{\rm A}}}} \right]{{\rm \ x\ 100}}$$
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$${{\rm EE\ }}\left({{\rm \% }} \right){{\rm \ =}}\left[{{{{{\rm A - B}}} \over {{\rm A}}}} \right]{{\rm \ x\ 100}}$$
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