Pharmacological Modulation of P2X Receptors

The following drugs were freshly prepared before use and administered intraperitoneally (ip): BBG (#B0770; Sigma Aldrich, St. Louis, MO, USA), a P2RX7/P2RX4 antagonist (Jiang et al., 2000 (link)), diluted in sterile isotonic saline and 2% tween 80, administered at 25 or 50 mg/kg/day doses (Csölle et al., 2013a (link); Carmo et al., 2014 (link)). Imipramine (IMI; #I7379; Sigma Aldrich, St. Louis, MO, USA), a tricyclic antidepressant, diluted in sterile isotonic saline, administered at 15 mg/kg/day dose (Stanquini et al., 2017 (link)). Chloral hydrate (Vetec) was used as a sedative for sample collection, at 5% concentration and administered at one ml/100g volume.