At 7 days after stroke, MCAO rats in each group were perfused with ice-cold PBS followed by 4% phosphate-buffered paraformaldehyde. IF was performed as described previously19 (link)
. The primary antibodies were: Mouse anti-CD31 (GB12063, Servicebio, China), rat anti-von-Willebrand-Factor(anti-vWF) (GB11020, Servicebio, China), The secondary antibodies used were: Cy3-labeled goat anti-mouse IgG (GB21301, Servicebio, China), FITC-labeled goat anti-rabbit IgG (GB22303l, Servicebio, China).
For quantification, three randomly selected high-power fields (HPFs; 400× or 200× for IF study) were analyzed in each section. The mean number of positively-stained cells per HPF for each animal was calculated.
. The primary antibodies were: Mouse anti-CD31 (GB12063, Servicebio, China), rat anti-von-Willebrand-Factor(anti-vWF) (GB11020, Servicebio, China), The secondary antibodies used were: Cy3-labeled goat anti-mouse IgG (GB21301, Servicebio, China), FITC-labeled goat anti-rabbit IgG (GB22303l, Servicebio, China).
For quantification, three randomly selected high-power fields (HPFs; 400× or 200× for IF study) were analyzed in each section. The mean number of positively-stained cells per HPF for each animal was calculated.
