Plasma Cytokine Analysis via Multiplex Immunoassay

In line with previously published procedures, plasma cytokine concentrations were analyzed using a multiplex immunoassay (High Sensitivity 5-Plex Mouse ProcartaPlex™ Panel; Thermo Fisher Scientific, Darmstadt, Germany) following the manufacturer's recommendations (18 (link)). Concentrations of plasma cytokines were quantified using the LiquiChip luminex 200 system (Qiagen, Hilden, Germany) and the Procartaplex-analyst 1.0 software (Affymetrix, eBioscience, San Diego, USA).

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Spielmann J., Naujoks W., Emde M., Allweyer M., Fänder J., Kielstein H., Quandt D, & Bähr I. (2021). The Impact of High-Fat Diet and Restrictive Feeding on Natural Killer Cells in Obese-Resistant BALB/c Mice. Frontiers in Nutrition, 8, 711824.

Publication 2021

5-Plex Mouse ProcartaPlex™ Panel; LiquiChip luminex 200 system Procartaplex-analyst 1.0 software

Cytokines Immunoassay Mouse Plasma Sensitivity

Corresponding Organization : Martin Luther University Halle-Wittenberg

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Plasma cytokine concentrations

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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