Monosaccharide Composition Analysis of EPS

Monosaccharide composition was determined according to the protocol described by Kamerling et al. [77 (link)] and modified by Montreuil et al. [78 ]. The EPS solution was firstly hydrolyzed for 4 h at 100 °C in methanol/HCl 3 N (Merck, Lyon, France). The obtained methyl glycosides were then converted to trimethylsilyl derivatives with the use of N,O-bis(trimethylsilyl)trifluoroacetamide and trimethylchlorosilane (BSTFA: TMCS) (Merck, Lyon, France) (99:1). Per-O-trimethylsilyl methyl glycosides formed were quantified using gas chromatography (GC-FID, Agilent Technologies, Les Ulis, France). The myo-inositol was used as the internal standard, as previously described [52 (link)].