For fixed samples, all images were captured using an Olympus FV1000 Laser Scanning Microscope with Becker and Hickel FLIM system and Olympus Fluoview FV1200 using FV10-ASW software (version 4.2). Foci counting was performed using FIJI (Image J version 2.0.0-rc-65/1.52a or 2.1.0) and the GDSC (FindFoci) ImageJ plugin60 (link). Only the foci that overlap with the DAPI staining were analysed. Live-cell images were captured using a Zeiss 880 inverted confocal microscope with Zen Black software (version 14.0.22.201). Images were captured every 4.5 min (Fig. 5 b) or 5 min (Fig. 5d ) for at least 6 h.
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