For p300, we chemically crosslinked and sonicated cells to generate fractionated genomic DNA. Chromatin immunoprecipitation was performed by using anti-p300 (sc-585, Santa Cruz Biotechnology). The DNA fragments were blunt-end ligated to the Illumina adaptors, amplified, and sequenced by using the Illumina Genome Analyzer II (Illumina, San Diego, CA). Sequence reads of 25 or 36 bps were obtained by using the Illumina Analysis Pipeline. Publically available ChIP-seq datasets are listed in Table S1 and were obtained from several published studies 12 (link)-15 (link),26 (link)-29 (link).
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ChIP-seq Analysis of p300 Binding
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Corresponding Organization :
Other organizations : National Institutes of Health, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Center for Cancer Research, National Cancer Institute, University of Connecticut
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Variable analysis
independent variables
- Chemical crosslinking of cells
- Sonication of cells to generate fractionated genomic DNA
- Chromatin immunoprecipitation using anti-p300 (sc-585, Santa Cruz Biotechnology)
- DNA fragments blunt-end ligated to Illumina adaptors
- DNA fragments amplified and sequenced using Illumina Genome Analyzer II
- Not explicitly mentioned
- No positive or negative controls were explicitly mentioned in the input protocol.
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