Culturing HEK293, HeLa, and HEK293 Cells

HEK293 Flp‐In TRex (Invitrogen), HeLa (Austin et al, 2017 (link)), and HEK293 (ATCC CRL‐1573) cells were maintained in DMEM supplemented with FBS (10% v/v), and penicillin/streptomycin (pen/strep) (1%). Cells were cultured in an incubator set to 37°C and 5% CO₂ and splitted when reaching confluency of ~70–90%, and regularly tested for mycoplasma.

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Austin S., Mekis R., Mohammed S.E., Scalise M., Wang W., Galluccio M., Pfeiffer C., Borovec T., Parapatics K., Vitko D., Dinhopl N., Demaurex N., Bennett K.L., Indiveri C, & Nowikovsky K. (2022). TMBIM5 is the Ca2+/H+ antiporter of mammalian mitochondria. EMBO Reports, 23(12), e54978.

Publication 2022

HEK293 Flp‐In TRex HEK293

Austin Cells Hela Mycoplasma Penicillin Streptomycin

Corresponding Organization :

Other organizations : Comprehensive Cancer Center Vienna, Medical University of Vienna, University of Veterinary Medicine Vienna, University of Calabria, University of Geneva, CeMM Research Center for Molecular Medicine, Austrian Academy of Sciences, Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies

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Variable analysis

independent variables

Cell lines used: HEK293 Flp‐In TRex (Invitrogen), HeLa (Austin et al., 2017), and HEK293 (ATCC CRL‐1573)

dependent variables

Not explicitly mentioned

control variables

Culture medium: DMEM supplemented with FBS (10% v/v) and penicillin/streptomycin (1%)
Incubator conditions: 37°C and 5% CO2
Cell culture: Cells were split when reaching confluency of ~70–90% and regularly tested for mycoplasma

controls

No positive or negative controls were explicitly mentioned in the provided information

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