Melanoma cell lines (A375P and SK-MEL-2) were obtained from either the Korean Cell Line Bank (KCLB; Seoul, Korea) or YOUAI Co., Ltd. (Suwon-Si, Gyeonggi-Do, Korea). The development of BRAF inhibitor-resistant A375P melanoma cells (A375P/Mdr) was previously described (Ahn and Lee, 2013 (link)). All of the cell lines were maintained at 37°C in DMEM supplemented with 10% FCS, penicillin-streptomycin, and glutamine. The A375P/Mdr cells were further propagated in growth medium containing 1 μM PLX4720. Before their use in the experiments, the A375P/Mdr cells were maintained in PLX4720-free culture medium and subcultured at least three times. For experimental purposes, the cells were cultured in 60-mm tissue culture dishes until they reached ∼80% confluency.
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Establishing BRAF-Inhibitor Resistant Melanoma Cell Lines
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Braf
Cell lines
Cells
Culture medium
Dishes
Glutamine
Korean
Melanoma
Penicillin
Plx4720
Streptomycin
Tissue
Corresponding Organization :
Other organizations : Korea Research Institute of Bioscience and Biotechnology, Incheon National University
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Variable analysis
independent variables
- BRAF inhibitor PLX4720
- Growth and proliferation of A375P/Mdr melanoma cells
- Cell culture conditions (37°C, DMEM supplemented with 10% FCS, penicillin-streptomycin, and glutamine)
- Cell confluency (approximately 80%)
- Parental A375P melanoma cells (without BRAF inhibitor resistance)
- SK-MEL-2 melanoma cells (non-resistant to BRAF inhibitors)
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