Fluorescence microscopy and live imaging were performed as described previously15 (link). Briefly, an FV1200-IX83 laser scanning confocal microscope with an oil-immersed 60 × objective (Olympus, Japan) was used for fluorescence microscopy. For live cell imaging, cells grown on a glass-bottomed dish were placed on a stage top incubator (Tokai Hit, Japan) that maintained a humidified atmosphere of 5% CO2 at 37 °C. Images were captured and analyzed using FLUOVIEW software (Olympus). Fluorescence intensity of a region of interest (ROI) was quantified using FLUOVIEW software.
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