Human cancer cell lines, SW620, H358, and H293T, were purchased from ATCC, UT89 was generated from a primary recurrent uterine serous tumor in the laboratory of Dr. Analisa DiFeo. SW620 and H358 were cultured in RPMI, H293T and UT89 were cultured in DMEM. All media was supplemented with 10% FBS (ThermoFisher, SH3007003) and 1% penicillin/streptomycin (GE Healthcare, SV30010). HMECs were obtained from Dr. Mark Jackson. Specimen 48R and derivatives were grown as described previously21 ,22 (link). HMEC derivatives were generated as previously described21 ,23 (link). All cells were grown in a humidified atmosphere containing 5% CO2. All cells lines underwent monthly testing for mycoplasma contamination (Lonza, LT07-710). AZD-6244 (Selleck Chemical, S1008) was dissolved in DMSO at a concentration of 80 mM and stored at −20°C (for in vitro studies). Okadaic Acid (OA) (P212121, LC-O-2220) was dissolved in DMSO at a concentration of 10 mM and stored at −20°C.
Protocol detail
Comprehensive Characterization of Cell Lines
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Atmosphere
Azd 6244
Cancer
Cells
Cells lines
Derivatives
Dmso
Human
Mycoplasma
Okadaic acid
Penicillin
Serous tumor
Streptomycin
Uterine
Corresponding Organization :
Other organizations : Case Western Reserve University, University of Michigan–Ann Arbor, University of Washington, Lunenfeld-Tanenbaum Research Institute, Sinai Health System, University of Turku, Åbo Akademi University, Turku Centre for Biotechnology, University of Virginia, University College London
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Variable analysis
independent variables
- AZD-6244 (Selleck Chemical, S1008)
- Okadaic Acid (OA) (P212121, LC-O-2220)
- Cell growth
- Cell viability
- Mycoplasma contamination
- Cell lines: SW620, H358, H293T, UT89, HMECs, Specimen 48R and derivatives
- Cell culture media: RPMI, DMEM
- Cell culture supplements: 10% FBS, 1% penicillin/streptomycin
- Cell culture conditions: 5% CO2, humidified atmosphere
- No positive controls were explicitly mentioned.
- No negative controls were explicitly mentioned.
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