Ensiling of Alfalfa Meal with Molasses and Saccharomyces

The whole part of AM plant was gathered from the semi-arid rangeland of Torbat-e Jam in July 2021. This area is 8184 square kilometers, from 60° 15´E to 60° 30´E, and 34° 35´N to 35° 47´N. In average, its height is 928 m above sea level. The samples were chapped with a laboratory chopper into pieces of about 2 cm. The chapped samples were ensiled in a 3.5 kg polyethylene mini-silos for 60 days. Fresh AM was ensiled in the mini-silos (3.5 kg) and treated with 1] no additive (control, AM1), 2] 5% molasses (AM2), 3] 10% molasses (AM3), 4] 1 × 10⁴ CFU (colony forming units)/g of fresh silage (AM4), 5] 1 × 10⁴ CFU of SC/g of fresh silage + 5% molasses (AM5), 6] 1 × 10⁴ CFU of SC/g of fresh silage + 10% molasses (AM6), 7] 1 × 10⁸ CFU of SC/g of fresh silage (AM7), 8] 1 × 10⁸ CFU of SC/g of fresh silage + 5% molasses (AM8), 9] 1 × 10⁸ CFU of SC/g of fresh silage + 10% molasses (AM9) for 60 days. Five replicates were considered for each treatment. Kimiazyme Company (Tehran, Iran) prepared the SC (8 × 10¹⁰ CFU/g of fresh material) with the trade name Zy-MOS Ultra.