Comprehensive Molecular Toolkit for Cellular Studies

For regents, CHX (Sigma, St Louis, MO, USA), MG132 (MedChemExpress, Monmouth Junction, NJ, USA), 3-MA (MedChemExpress), CHQ (MedChemExpress), Bort (MedChemExpress), and IFNα (Sigma) were used to treat cells. For plasmids, ISG15 and 6PGL expression plasmids were bought from Origene (Beijing, China). LentiCRISPR v2 based constructs were used for knockout ISG15, UbCH8, HERC5, 6PGL, SMADs, TEADs. pGL4.21 vector was used to construct a 6PGL promoter-luciferase vector. YAP^K280R-HA, YAP^K321R-HA, YAP^K497R-HA, 6PGL^Mut-P1, 6PGL^Mut-P2, 6PGL^Mut-P3, and 6PGL^Mut-P1+P2+P3 mutant plasmids were constructed using overlapping PCR. YAP, ATG5, PSMB5, and βTrCP knockout constructs, YAP^WT-HA, YAP^FLAG, SMAD2, TEAD4, RUNX2, TFCP2, P73, pUAS-Luc/TEAD-Gal4 plasmids were acquired from previous studies [15 (link), 31 (link), 43 (link), 49 (link), 50 (link)]. The primers are listed in Supplementary Table 1.