Rapid Diagnosis of C. difficile Infection

Stool samples or rectal swabs were obtained from each patient within 48 h of hospital admission. Toxigenic C. difficile carriers were identified with a real-time PCR assay, which simultaneously detects toxin A (TcdA enterotoxin, encoded by tcdA) and toxin B (TcdB cytotoxin, encoded by tcdB) (AdvanSure CD Real-Time PCR Kit; LG Life Science, Seoul, Korea). Enzyme-linked immunosorbent assay (ELISA) (C. DIFFICILE TOX A/B II, TECHLAB, USA) was used to evaluate the stool samples for toxin A and B production.

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Lee K.B., Lee M., Suh J.W., Yang K.S., Chung Y., Kim J.Y., Kim S.B., Sohn J.W, & Yoon Y.K. (2023). Clinical prediction rule for identifying older patients with toxigenic clostridioides difficile at the time of hospital admission. BMC Geriatrics, 23, 127.

Publication 2023

Assay C a b Cytotoxin Enterotoxin Enzyme linked immunosorbent assay Patient Real time pcr Rectal Stool Tcdb Toxin

Corresponding Organization :

Other organizations : Korea University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Toxigenic C. difficile carrier status
Toxin A (TcdA enterotoxin, encoded by tcdA) production
Toxin B (TcdB cytotoxin, encoded by tcdB) production

control variables

None explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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