To evaluate the proliferative capacity of activated immune cells, SEB-activated MNCs from lungs or in vitro SEB-activated splenocytes were treated with thymidine (methyl-3H; PerkinElmer Health Sciences, United States) isotope at a concentration of 1µCi/well and incubated for 16 h to and then radioactivity was measured using a liquid-scintillation counter (MicroBeta TriLux; PerkinElmer, United States).
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