Immunohistochemical and Osteoclast Analysis in Arthritis

Immunohistochemistry included rabbit monoclonal anti-TNFα (diluted 1:1,000; HP8001, rabbit IG; Hycult Biotechnology BV, Uden, the Netherlands). The section of TNFα was developed with Dako EnVision + Dual Link System-HRP (DAB+) kit (Dako, Glostrup, Denmark). Counterstaining involved hematoxylin, and rabbit IgG served as negative control. Briefly, three sections from each femoral condyle and tibial plateau were scored by two investigators blinded to origin of the samples. Total and positively stained chondrocytes were counted and reported as percentages, where 100% was the maximum score.41 (link)
Osteoclast activity was observed by tartrate-resistant acid phosphatase (TRAP) staining with leukocyte acid phosphatase kits (Sigma-Aldrich Chemical Co.) following the manufacturer’s instructions. Nuclei were counterstained with Gill’s hematoxylin (Sigma-Aldrich Chemical Co.), and averages of TRAP-positive osteoclasts were calculated.25 (link)

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Shi Q., Rondon-Cavanzo E.P., Dalla Picola I.P., Tiera M.J., Zhang X., Dai K., Benabdoune H.A., Benderdour M, & Fernandes J.C. (2018). In vivo therapeutic efficacy of TNFα silencing by folate-PEG-chitosan-DEAE/siRNA nanoparticles in arthritic mice. International Journal of Nanomedicine, 13, 387-402.

Publication 2018

Dako EnVision + Dual Link System-HRP (DAB+) kit leukocyte acid phosphatase kits Gill’s hematoxylin

Acid phosphatase Chondrocytes Condyle Femoral Gill Hematoxylin Immunohistochemistry Leukocyte Nuclei Osteoclasts Rabbit Tartrate resistant acid phosphatase Tibial Tnfα

Corresponding Organization : Université de Montréal

Other organizations : Shanghai Jiao Tong University, Shanghai Ninth People's Hospital

Top 5 similar protocols

Variable analysis

independent variables

Immunohistochemistry for TNFα with rabbit monoclonal anti-TNFα antibody
TRAP staining for osteoclast activity

dependent variables

Percentage of total and positively stained chondrocytes
Average of TRAP-positive osteoclasts

control variables

Sections from each femoral condyle and tibial plateau
Samples were scored by two investigators blinded to their origin
Rabbit IgG served as a negative control for the immunohistochemistry

controls

Positive control: Immunohistochemistry for TNFα using rabbit monoclonal anti-TNFα antibody
Negative control: Rabbit IgG for immunohistochemistry

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!