Preparing Hydrogel Substrates for Cell Culture

Hydrogels are prepared as described in the previous work[35 (link)]. Briefly, for elastic substrate 8% acrylamide and 0.1% bis-acrylamide (Bio-Rad), 2% N-hydroxysuccinimide (dissolved in DMSO) (Sigma), 0.375 % 3-Aminopropylsilyl (Thermo Fisher Scientific), 0.125% tetramethylethylenediamine (Millipore Sigma) added into water to prepare elastic gel of stiffness 5kPa (G’=5kPa and G” =0Pa) and for viscoelastic gel (G’ = 5kPa, G” =600Pa) 14.3 % linear acrylamide (5%stock) and 0.15% bis-acrylamide (instead 0.1%) added to the same protocol as elastic substrate recipe (Fig 1(a)). Gels are laminated with (100 μg/ml) collagen type I (Corning) by incubation overnight at 4 °C.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Mandal K., Gong Z., Rylander A., Shenoy V.B, & Janmey P.A. (2020). Opposite responses of normal hepatocytes and hepatocellular carcinoma cells to substrate viscoelasticity. Biomaterials science, 8(5), 1316-1328.

Publication 2020

bis-acrylamide N-hydroxysuccinimide 3-Aminopropylsilyl tetramethylethylenediamine collagen type I

Acrylamide Collagen type i Dmso Gels Hydrogels Tetramethylethylenediamine

Corresponding Organization : Philadelphia University

Top 5 similar protocols

Variable analysis

independent variables

Gel composition (8% acrylamide and 0.1% bis-acrylamide for elastic substrate, 14.3% linear acrylamide and 0.15% bis-acrylamide for viscoelastic gel)

dependent variables

Gel stiffness (G' and G'')

control variables

N-hydroxysuccinimide (2% dissolved in DMSO)
3-Aminopropylsilyl (0.375%)
Tetramethylethylenediamine (0.125%)
Collagen type I coating concentration (100 μg/ml)
Incubation temperature (4 °C)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!