The percentages of T lymphocyte subpopulations from spleen were measured with flow cytometric analysis [20 (link)]. Splenocyte suspensions (1 × 106 cells/mL) were stained with anti-mouse CD3e-PE-cy5, CD4-PE, and CD8a-FITC antibodies (eBioscience, San Diego, CA, USA) for 20 min at room temperature in the dark. Then cells were washed with PBS to remove the excess stains. Each sample was suspended in 500 μL of PBS and analyzed with a flow cytometer (FC500, Beckman Counter, USA). The data were acquired by gating on CD3+CD4+ or CD3+CD8+ cell population.
Free full text: Click here
