Three days after cRNA injection, we injected oocytes with 23 nl nuclease-free H2O or 50 ng of commercially purchased CA (C3934, Sigma, derived from bovine erythrocytes) dissolved in 23 nl nuclease-free H2O. Injection volume and bCA quantity replicate the parameters used by the Deitmer laboratory (Becker & Deitmer, 2007 (link); Schueler et al., 2011 (link)).
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Carbonic Anhydrase Injection in Oocytes
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Variable analysis
independent variables
- Injection of 23 nl nuclease-free H2O
- Injection of 50 ng of commercially purchased CA (C3934, Sigma, derived from bovine erythrocytes) dissolved in 23 nl nuclease-free H2O
- Not explicitly mentioned
- Injection volume (23 nl)
- Quantity of bCA (50 ng)
- Replicated parameters used by the Deitmer laboratory (Becker & Deitmer, 2007; Schueler et al., 2011)
- Positive control: Injection of 50 ng of commercially purchased CA (C3934, Sigma, derived from bovine erythrocytes) dissolved in 23 nl nuclease-free H2O
- Negative control: Injection of 23 nl nuclease-free H2O
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