Generation of Transgenic ERK Mouse Lines

Transgenic mouse lines carrying the ERK2 floxed alleles (Samuels et al., 2008 (link)) as well as ERK1 null mice were generated previously (Nekrasova et al., 2005 ; Samuels et al., 2008 (link)). Emx1-Cre and Camk2a-Cre mouse lines were obtained from Jackson Laboratories. All mice were maintained on C57/B6 background. Animals were housed in the Case Western Reserve University (CWRU) Animal Resource Center (ARC) on a 12 hour light-dark cycle, with regular cage changes and ad libitum food and water.

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Vithayathil J., Pucilowska J., Friel D, & Landreth G.E. (2017). Chronic impairment of ERK signaling in glutamatergic neurons of the forebrain does not affect spatial memory retention and LTP in the same manner as acute blockade of the ERK pathway. Hippocampus, 27(12), 1239-1249.

Publication 2017

Emx1-Cre Camk2a-Cre

Alleles Animal Erk1 Erk2 Food Mice Null mice Transgenic mouse

Corresponding Organization : Case Western Reserve University

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Variable analysis

independent variables

Transgenic mouse lines carrying the ERK2 floxed alleles
ERK1 null mice
Emx1-Cre mouse lines
Camk2a-Cre mouse lines

dependent variables

Not explicitly mentioned

control variables

C57/B6 background
12 hour light-dark cycle
Regular cage changes
Ad libitum food and water

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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