Western Blotting Protein Analysis

Western blotting was carried out as previously described5 (link) using rabbit antibodies against the following proteins: IR (1:800), IRS-1 (1:800), p-AKT (1:800), GSK-3β (S9) (all 1:800), or glyceraldehyde phosphate dehydrogenase (GAPDH) (1:10,000) (all from Bioworld, San Diego, CA, USA); GAPDH was used as an internal reference standard. Secondary antibodies were peroxidase-conjugated goat anti-rabbit IgG (H + L) (ZSGB-BIO, Beijing, China).

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Cui B., Gai Z., She X., Wang R, & Xi Z. (2016). Effects of chronic noise on glucose metabolism and gut microbiota–host inflammatory homeostasis in rats. Scientific Reports, 6, 36693.

Publication 2016

IRS-1 p-AKT GAPDH) peroxidase-conjugated goat anti-rabbit IgG (H + L)

Anti igg Antibodies Glyceraldehyde phosphate dehydrogenase Goat Irs 1 Peroxidase Proteins Rabbit

Corresponding Organization :

Other organizations : Shandong First Medical University

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Variable analysis

independent variables

Antibodies used for Western blotting: IR (1:800), IRS-1 (1:800), p-AKT (1:800), GSK-3β (S9) (1:800), and GAPDH (1:10,000)

dependent variables

Protein expression levels of IR, IRS-1, p-AKT, GSK-3β (S9), and GAPDH

control variables

Secondary antibody: peroxidase-conjugated goat anti-rabbit IgG (H + L)
GAPDH used as an internal reference standard

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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