Cytokine Profiling in Patient Sera

Sera were collected from patients at baseline and on days 1, 2, 3, and 7. IL-6, IL-2, IFN-γ, and tumor necrosis factor alpha (TNF-α) concentrations were measured with ELISA commercial kits (DRG Diagnostics, Marburg, Germany), using an automated immunoassay analyzer (Stat Fax, Los Angeles, California, USA). Levels of 48 different human cytokines and chemokines in culture supernatants were determined using Bio-Plex kits (BioRad, Hercules, California, USA), as previously described.21 (link)

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Ishihara M., Kitano S., Kageyama S., Miyahara Y., Yamamoto N., Kato H., Mishima H., Hattori H., Funakoshi T., Kojima T., Sasada T., Sato E., Okamoto S., Tomura D., Nukaya I., Chono H., Mineno J., Kairi M.F., Diem Hoang Nguyen P., Simoni Y., Nardin A., Newell E., Fehlings M., Ikeda H., Watanabe T, & Shiku H. (2022). NY-ESO-1-specific redirected T cells with endogenous TCR knockdown mediate tumor response and cytokine release syndrome. Journal for Immunotherapy of Cancer, 10(6), e003811.

Publication 2022

Bio-Plex kits

Chemokines Cytokines Diagnostics Elisa Human Ifn γ Immunoassay Patients Sera Tnf α

Corresponding Organization : Mie University

Other organizations : Mie University Hospital, Japanese Foundation For Cancer Research, The Cancer Institute Hospital, Tokyo National Hospital, Aichi Medical University, Nagoya Medical Center, National Hospital Organization, Keio University, National Cancer Center Hospital East, Kanagawa Prefectural Hospital Organization, Tokyo Medical University, Takara (Japan), Nagasaki University

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Variable analysis

independent variables

Time of sample collection (baseline, days 1, 2, 3, and 7)

dependent variables

IL-6 concentration
IL-2 concentration
IFN-γ concentration
Tumor necrosis factor alpha (TNF-α) concentration
Levels of 48 different human cytokines and chemokines

control variables

ELISA commercial kits (DRG Diagnostics, Marburg, Germany)
Automated immunoassay analyzer (Stat Fax, Los Angeles, California, USA)
Bio-Plex kits (BioRad, Hercules, California, USA)

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

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