Place Aversion Behavioral Assay

Place aversion was tested in a custom-designed plexiglass chamber with a layer of corn cob bedding (18 (link), 21 (link)). Each arm of the two-arm V-maze was 10 cm wide, 33 cm long, and 10 cm high with a neutral area between the two arms. The signal was generated in one arm only by tethering the designated arm with a double loop antenna. The signal was attenuated to allow for complete local field coverage and precise control of μ-ILED device power within the designated arm. Each mouse was placed in the neutral area of the chamber, and activity was continuously recorded using a video camera for a period of 20 min. “Time in chamber” and heat maps were generated for data using EthoVision software (Noldus, Leesburg, VA, USA). The μ-ILED device was instantaneously and automatically switched to the “ON” state each time the mouse entered into the double-loop antenna–equipped arm. Similarly, the μ-ILED device was automatically switched to the “OFF” state each time the mouse exited the antenna-equipped arm. Software and hardware for generating the signal were from NeuroLux Inc. (Champaign, IL, USA).

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Zhang Y., Mickle A.D., Gutruf P., McIlvried L.A., Guo H., Wu Y., Golden J.P., Xue Y., Grajales-Reyes J.G., Wang X., Krishnan S., Xie Y., Peng D., Su C.J., Zhang F., Reeder J.T., Vogt S.K., Huang Y., Rogers J.A, & Gereau RW I.V. (2019). Battery-free, fully implantable optofluidic cuff system for wireless optogenetic and pharmacological neuromodulation of peripheral nerves. Science Advances, 5(7), eaaw5296.

Publication 2019

EthoVision software

Corn Device Maps Maze Mouse Plexiglass

Corresponding Organization : Neurological Surgery

Other organizations : Shenzhen University, Georgia Institute of Technology

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Variable analysis

independent variables

Presence or absence of the signal generated in one arm of the two-arm V-maze (i.e., the signal was generated in one arm only)

dependent variables

Time spent in the chamber
Heat maps of mouse activity

control variables

Size of the two-arm V-maze chamber (10 cm wide, 33 cm long, and 10 cm high with a neutral area between the two arms)
Layer of corn cob bedding in the chamber (18 cm and 21 cm depth)
Video recording duration (20 minutes)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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