Phenotypic Analysis of Human PBMC Subsets
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Corresponding Organization :
Other organizations : Sorbonne Université, Inserm, Centre National de la Recherche Scientifique, Sanford Research, University of South Dakota, National Cancer Institute
Variable analysis
- Conjugated anti-human monoclonal antibodies (mAb)
- FITC–conjugated anti-CD21
- Energy-coupled dye–conjugated anti-CD19
- APC–conjugated anti-IgM
- PE–Cy7–conjugated anti-CD27
- PE-conjugated anti–FCRL-1
- PE-conjugated anti–FCRL-2
- PE-conjugated anti–FCRL-3
- PE-conjugated anti–FCRL-4
- PE-conjugated anti–FCRL-5
- PE-conjugated anti–FCRL-4 mAb (clone 413D12) from BioLegend
- PE-conjugated IgG1 (Beckman Coulter) as an isotype control
- Flow cytometry data
- Staining of PBMCs
- Positive controls: Reference antibodies for FCRL proteins 1-5 (CD307a-e) from previous international human leukocyte differentiation antigens workshops
- Negative control: PE-conjugated IgG1 (Beckman Coulter) as an isotype control
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