Protocol detail

Pharmacological Agents for Cell Studies

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Pirfenidone (10 to 300 μg/ml [71 (link), 116 –118 (link)]) was obtained from Chemietek. AraC (1 to 10 μM [119 (link), 120 ]), LY2228820 (0.03 to 2 μM [68 (link), 69 (link), 121 (link), 122 (link)]), BIRB796 (BIRB, 5 μM [70 (link), 123 (link)–126 (link)]), and JNK-IN-8 (1 μM [127 (link)] were from Selleckchem. KU55933 (10 μM [128 (link)], tested but was toxic for the cells tested), BAY 11-7082 (10 μM [78 (link)]), and D-luciferin were from Cayman Chemical and doxorubicin (10 to 500 nM [129 (link)]) was from Tocris Bioscience.

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Lee S., Micalizzi D., Truesdell S.S., Bukhari S.I., Boukhali M., Lombardi-Story J., Kato Y., Choo M.K., Dey-Guha I., Ji F., Nicholson B.T., Myers D.T., Lee D., Mazzola M.A., Raheja R., Langenbucher A., Haradhvala N.J., Lawrence M.S., Gandhi R., Tiedje C., Diaz-Muñoz M.D., Sweetser D.A., Sadreyev R., Sykes D., Haas W., Haber D.A., Maheswaran S, & Vasudevan S. (2020). A post-transcriptional program of chemoresistance by AU-rich elements and TTP in quiescent leukemic cells. Genome Biology, 21, 33.

Publication 2020

AraC LY2228820 JNK-IN-8 KU55933 BAY 11-7082 D-luciferin doxorubicin

Arac Bay 11 7082 Birb796 Cayman Cells Doxorubicin Ku55933 Luciferin Ly2228820 Pirfenidone

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Pirfenidone (10 to 300 μg/ml)
AraC (1 to 10 μM)
LY2228820 (0.03 to 2 μM)
BIRB796 (BIRB, 5 μM)
JNK-IN-8 (1 μM)
KU55933 (10 μM)
BAY 11-7082 (10 μM)
Doxorubicin (10 to 500 nM)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Doxorubicin (10 to 500 nM)

negative controls

KU55933 (10 μM) (was toxic for the cells tested)

Annotations

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