Western Blot Antibody Characterization

For Western blotting, anti-α-tubulin (T6199, 1:5000 dilution) and anti-Flag (M2) (F3165-5MG, 1:5000 dilution) antibodies were obtained from Sigma-Aldrich. Anti-Myc (sc-40, 1:500 dilution) and anti-GST antibodies (sc-138, 1:1000 dilution) were purchased from Santa Cruz Biotechnology. Anti-phospho-YAP (S127) (4911S, 1:1000 dilution) and anti-phospho-LATS1 (Thr1079) (8654S, 1:1000 dilution) antibodies were purchased from Cell Signaling Technology. Anti-hemagglutinin (HA) monoclonal antibody (901514, 1:2000 dilution) was obtained from BioLegend. Anti-Thiophosphate ester antibody (ab92670, 1:1000 dilution) was purchased from Abcam. The YAP and MBP polyclonal antibodies were generated as previously described^{57 (link),61 (link)}. ATP-γ-S kinase substrate (ab138911) and p-Nitrobenzyl mesylate (ab138910) were obtained from Abcam. For immunostaining, an anti-YAP (sc-101199, 1:200 dilution) monoclonal antibody was purchased from Santa Cruz Biotechnology. Anti-Flag polyclonal antibody (F7425, 1:5000 dilution) was obtained from Sigma-Aldrich.

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Chen Y., Han H., Seo G., Vargas R.E., Yang B., Chuc K., Zhao H, & Wang W. (2020). Systematic analysis of the Hippo pathway organization and oncogenic alteration in evolution. Scientific Reports, 10, 3173.

Publication 2020

anti-α-tubulin (T6199, anti-Flag (M2) (F3165-5MG Anti-Myc (sc-40 Anti-phospho-YAP (S127) (4911S, ab138911 p-Nitrobenzyl mesylate ab138910 anti-YAP (sc-101199,

Anti antibodies Anti antibody Antibodies Atp kinase Dilution Ester Hemagglutinin Lats1 Mesylate Monoclonal antibody Thiophosphate α tubulin

Corresponding Organization :

Other organizations : University of California, Irvine, Wuhan University

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Variable analysis

independent variables

Western blotting antibody dilutions

dependent variables

Protein expression and phosphorylation levels

control variables

Antibodies used for Western blotting (anti-α-tubulin, anti-Flag, anti-Myc, anti-GST, anti-phospho-YAP, anti-phospho-LATS1, anti-HA, anti-Thiophosphate ester, anti-YAP, anti-Flag polyclonal)

controls

Positive controls: YAP and MBP polyclonal antibodies
Negative controls: Not explicitly mentioned

Annotations

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